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- Elabscience
- E-AB-F1103L
- 湖北武汉
- 2025年07月14日
- FCM
- Armenian Hamster
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Elab Fluor® 488 Anti-Mouse CD3ε Antibody流式抗体[145-2C11]_货号:E-AB-F1103L
- 抗体英文名：
Elab Fluor® 488 Anti-Mouse CD3ε Antibody[145-2C11]_货号:E-AB-F1103L
- 应用范围：
FCM
- 宿主：
Armenian Hamster
- 适应物种：
Mouse
- 保质期：
12个月
- 目录编号：
E-AB-F1103L
- 级别：
科研
- 库存：
999
- 供应商：
武汉伊莱瑞特生物科技股份有限公司
- 标记物：
Elab Fluor<sup>®</sup> 488
- 克隆性：
Monoclonal
- 保存条件：
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
- 形态：
液体
- 亚型：
Armenian Hamster IgG
- 规格：
100Tests/50Tests/100Tests×2
| 规格： | 100Tests | 产品价格： | ￥1032.0 |
|---|---|---|---|
| 规格： | 50Tests | 产品价格： | ￥660.0 |
| 规格： | 100Tests×2 | 产品价格： | ￥1860.0 |
货号：E-AB-F1103L
| Background |
CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
|
| Alternate Names |
T-cell surface glycoprotein CD3 epsilon chain;CD3E;T-cell surface antigen T3/Leu-4 epsilon chain;CD3e;CD3E;T3E;
|
| Swissprot |
P22646
|
| Gene ID |
12501
|
| Clone No |
145-2C11
|
| Host |
Armenian Hamster
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Armenian Hamster IgG
|
| Conjugation |
Elab Fluor<sup><sup>®</sup></sup> 488
|
| Conjugation Information |
Elab Fluor® 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520 nm (e.g., a 525/40 nm bandpass filter).
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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每年引用文献数量持续增长，保持逐年30%+高增长率，可以预见，Elabscience产品将帮助更多科研工作者发表高质量SCI论文。
文献详情查看>>>>
分别利用 CD3 与 CD28 的功能学抗体，以及 CD3/CD28 Streptamer® 激活扩增 T 细胞的实验步骤与实验结果。 CD3/CD28抗体法 1、实验步骤： 1.1 抗体包被 用无菌 PBS 将 anti-mouse CD3 抗体（克隆号：145-2C11）稀释至 5μg/ml，稀释后的抗体加入到 24 孔板中，每孔 400μl，4°C 包被过夜。（注：板子在加入抗体之前，先用无菌 PBS 润洗 2-3 遍，避免干孔）； 1.2 小鼠脾脏淋巴细胞分离（次日） 1. 将 70μm
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
volume of Dynabeads taken from the vial (step 2). 2. Activation of Mouse T Cells 1) Start with 8 × 104 purified T cells in 100-200 µl medium in a 96-well tissue culture plate. 2) Add 2 µl Dynabeads Mouse T-Activator CD3/CD
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