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- 湖北武汉
- E-AB-F1029G
- 2025年07月16日
- FCM
- Rat
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
Rat IgG2a, κ
- 形态：
液体
- 保存条件：
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
- 克隆性：
Monoclonal
- 标记物：
PE/Cyanine5
- 适应物种：
Mouse
- 保质期：
12个月
- 目录编号：
E-AB-F1029G
- 级别：
科研
- 库存：
999
- 供应商：
武汉伊莱瑞特生物科技股份有限公司
- 宿主：
Rat
- 应用范围：
FCM
- 抗体英文名：
PE/Cyanine5 Anti-Mouse CD122/IL-2RB Antibody[5H4]_货号:E-AB-F1029G
- 抗体名：
PE/Cyanine5 Anti-Mouse CD122/IL-2RB Antibody流式抗体[5H4]_货号:E-AB-F1029G
- 规格：
100Tests/50Tests/100Tests×2
| 规格： | 100Tests | 产品价格： | ￥1032.0 |
|---|---|---|---|
| 规格： | 50Tests | 产品价格： | ￥660.0 |
| 规格： | 100Tests×2 | 产品价格： | ￥1860.0 |
货号：E-AB-F1029G
| Background |
CD122 is a 70-75 kD IL-2 receptor β chain also known as IL-2Rβ, which is also shared by the IL-15 receptor. It is constitutively expressed by NK cells and at lower levels by T cells, B cells, monocytes, and macrophages. The IL-2Rβ chain can combine with either the common γ subunit (γc, CD132) alone or with the γc subunit and the IL-2Rα subunit (CD25) to generate intermediate or high affinity IL-2 receptor complexes, respectively. CD122 expression levels can be upregulated by activation. The 5H4 antibody does not block IL-2 binding to the IL-2 receptor. CD122 is expressed on murine, but not human, CD8+ Tregs involved in the maintenance of T cell homeostasis.
|
| Alternate Names |
Interleukin-2 receptor subunit beta;Il2rb;IL-2 receptor subunit beta; IL-2R subunit beta;IL-2RB;High affinity IL-2 receptor subunit beta;p70-75;CD122;
|
| Swissprot |
P16297
|
| Gene ID |
16185
|
| Clone No |
5H4
|
| Host |
Rat
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Rat IgG2a, κ
|
| Isotype Control |
PE/Cyanine5 Rat IgG2a, κ Isotype Control[2A3]
|
| Conjugation |
PE/Cyanine5
|
| Conjugation Information |
PE/Cyanine5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 670 nm (e.g., a 690/50 nm bandpass filter).
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献详情查看>>>>
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a
Intracellular Cytokine Staining Protocol
)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble) 5hrMonensinJES6-5H4IL-4mouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble) 5hrMonensinBVD6-24G2IL-6mouse
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
antibody; (5) substrate is added, and is converted by enzyme to detectable form (e.g., color change into yellow). (B ) Competitive ELISA. (1) Plate is coated with a capture antibody; (2) sample is added, and any antigen present binds to capture antibody
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