- ￥1700 - 4000
- GeneTex
- 美国
- GTX105255
- 2025年07月12日
- WB, ICC/IF, IHC-P, IP
- Rabbit
- Human, Mouse, Rat, Zebrafish
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human PPP1A. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Zebrafish
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX105255
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IP
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Based on sequence homology, this antibody may cross react with PPP1CB or PPP1CC proteins.
- 抗体英文名：
PPP1A antibody
- 抗体名：
PPP1A 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
WB to detect PP1 alpha from U2OS cells treated with control siRNA or PP1 alpha siRNA, using GTX105255 at 1:1000 dilution. Nuclear matrix protein p84 is a loading control, blotted with p84 antibody (clone 5E10) GTX70220 at 1:5000 dilution. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
PPP1A antibody detects PPP1A protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
PPP1A stained by PPP1A antibody (GTX105255) diluted at 1:165.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
PPP1A antibody detects PPP1A protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PPP1A antibody (GTX105255) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using PPP1A antibody (GTX105255) at 1:300 dilution.
Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PPP1A antibody (GTX105255) diluted at 1:500.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PPP1A antibody (GTX105255) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Confocal immunofluorescence analysis (Olympus FV10i) of PFA-fixed HeLa, using PPP1A(GTX105255) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.
Immunohistochemical analysis of paraffin-embedded SAS xenograft, using PPP1A(GTX105255) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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文献和实验Yang J et al., Exp Ther Med 2023 (PMID:36911368)
Noguchi Y et al., iScience 2021 (PMID:34337357)
Liu CY et al., Br J Haematol 2017 (PMID:28340282)
Wei R et al., Mol Biol Cell 2011 (PMID:21832156)
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
General comments: Antibodies, like most proteins, do not like to be freeze-thawed. Avoid repetitive freezing of your solution. The best way to store your antibody is to keep a high protein concentration (>1 mg/ml), add some protease
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