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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human Glycerol kinase. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX102297
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF
- 浓度:
0.28 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Glycerol kinase
- 抗体英文名:
Glycerol kinase antibody [N3C2], Internal
- 抗体名:
Glycerol kinase 抗体 [N3C2], Internal
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Glycerol kinase antibody [N3C2], Internal detects Glycerol kinase protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: Glycerol kinase protein stained by Glycerol kinase antibody [N3C2], Internal (GTX102297) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX102297 diluted at 1:500
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文献和实验PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
affinity) and the other is an IP kinase reaction. Substrate/Antibody, Lysate Interaction: 1. For GST c-Jun substrate interaction the quantity of protein on the beads is variable with the batch made (make fresh at least once a week). Generally
Phosphatidylinositol 4-Kinase Assay in Ovarian Carcinoma Cells
Membrane-constituting phospholipids include glycerol phospholipids such as phos- phatidylcholine and phosphatidylinositol (PI) and sphingolipids. Recently, signal transduction starting from hydrolysis of these phospholipids have attracted
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