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B-Raf antibody [N2C1], Interna

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  • ¥1700 - 4000
  • GeneTex已认证
  • 美国
  • 2025年07月13日
  • WB, IHC-P, IP
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Recombinant protein encompassing a sequence within the center region of human B-Raf. The exact sequence is proprietary.

    • 亚型

      IgG

    • 形态

      Liquid

    • 保存条件

      Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

    • 克隆性

      Polyclonal

    • 标记物

      Unconjugated

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      12 months from the shipping date of the product. 

    • 抗原来源

      Human

    • 目录编号

      GTX100913

    • 级别

      Primary Antibodies

    • 库存

      Available

    • 供应商

      GeneTex

    • 宿主

      Rabbit

    • 应用范围

      WB, IHC-P, IP

    • 浓度

      0.21 mg/ml (Please refer to the vial label for the specific concentration.)

    • 靶点

      B-Raf

    • 抗体英文名

      B-Raf antibody [N2C1], Internal

    • 抗体名

      B-Raf 抗体 [N2C1], Internal

    • 规格

      100 μl/25 μl

    规格:100 μl产品价格:¥4000.0
    规格:25 μl产品价格:¥1700.0
    产品细节图片1

    B-Raf antibody [N2C1], Internal detects B-Raf protein at cytosol on mouse testis by immunohistochemical analysis.
    Sample: Paraffin-embedded mouse testis.
    B-Raf antibody [N2C1], Internal (GTX100913) dilution: 1:500.

    Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

    产品细节图片2

    B-Raf antibody [N2C1], Internal immunoprecipitates B-Raf protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of B-Raf protein expressing HepG2 cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of B-Raf by 3 μg of B-Raf antibody [N2C1], Internal (GTX100913) 10% SDS-PAGE The immunoprecipitated B-Raf protein was detected by B-Raf antibody [N2C1], Internal (GTX100913) diluted at 1:500. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

    产品细节图片3

    Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [N2C1], Internal (GTX100913) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

    产品细节图片4

    B-Raf antibody [N2C1], Internal detects B-Raf protein by western blot analysis. Non-transfected (-) and B-Raf-transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [N2C1], Internal (GTX100913) diluted at 1:5000.

    产品细节图片5

    B-Raf antibody [N2C1], Internal detects B-Raf protein at cytoplasm by immunohistochemical analysis.
    Sample: Paraffin-embedded human breast carcinoma.
    B-Raf stained by B-Raf antibody [N2C1], Internal (GTX100913) diluted at 1:500.
    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

    产品细节图片6

    B-Raf antibody [N2C1], Internal detects B-Raf protein by western blot analysis.
    A. 30 μg PC-12 whole cell extract
    7.5 % SDS-PAGE
    B-Raf antibody [N2C1], Internal (GTX100913) dilution: 1:5000

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    图标文献和实验
    该产品被引用文献
    Application Reference
    Rubin B et al., Cancer Invest 2015 (PMID:26536286)
    Tsuji S et al., Oncol Lett 2021 (PMID:33376545)
    Tateishi K et al., Acta Neuropathol Commun 2020 (PMID:32811569)
    Sase H et al., Mol Cancer Ther 2018 (PMID:30045926)
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    文献支持
    B-Raf antibody [N2C1], Internal
    ¥1700 - 4000