Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human p53. The exact sequence is proprietary.
亚型:
IgG
形态:
Liquid
保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Polyclonal
标记物:
Unconjugated
适应物种:
Human, Mouse
保质期:
12 months from the shipping date of the product.
抗原来源:
Human
目录编号:
GTX100629
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Rabbit
应用范围:
WB, ICC/IF, IHC-P, IP
浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
靶点:
p53
抗体英文名:
p53 antibody [N1], N-term
抗体名:
p53 抗体 [N1], N-term
规格:
100 μl/25 μl
规格:
100 μl
产品价格:
¥4000.0
规格:
25 μl
产品价格:
¥1700.0
p53 antibody [N1], N-term detects p53 protein at nucleus by immunofluorescent analysis. Samples: HCT 116 cells mock (left) and treated with 30 μM Cisplatin for 24 hrs (right) were fixed in 4% PFA at RT for 15 min. Green: p53 protein stained by p53 antibody [N1], N-term (GTX100629) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Confocal immunofluorescence analysis (Olympus FV10i) of PFA-fixed U2OS, using p53(GTX100629) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2.5 ug of anti-p53 antibody (GTX100629). (A) Input, 20ug of HCT116 lysates. The precipitated protein was detected by GTX100629 diluted at 1:10000. EasyBlot anti-Rabbit IgG Kit (GTX225856-01) was used in Western blot.
Immunohistochemical analysis of paraffin-embedded U373 xenograft, using p53(GTX100629) antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [N1], N-term (GTX100629) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Application Reference
Chen YJ et al., Life Sci 2013 (PMID:23624232)
Alhanouf A Aljohani et al., Toxics 2022 (PMID:35622687)
Doddapaneni R et al., Oncotarget 2019 (PMID:30728899)
Mlcochova P et al., EMBO J 2017 (PMID:29084722)
Abdi J et al., Blood Cancer J 2013 (PMID:23727662)