- ￥1700 - 4000
- GeneTex
- 美国
- GTX117997
- 2025年07月15日
- WB, ICC/IF, IHC-P, IP, ChIP assay
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
The immunogen used to generate this antibody corresponds to V5 tag
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 保质期：
12 months from the shipping date of the product.
- 目录编号：
GTX117997
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IP, ChIP assay
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
V5 tag
- 抗体英文名：
V5 tag antibody
- 抗体名：
V5 tag 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
V5 tag recombinant protein was immunoprecipitated from HeLa transfected cell lysate by using V5 tag antibody (GTX117997). The precipitated V5 tag recomabinant was detected by GTX117997 diluted at 1:1000. A: Input B: IP by V5 tag antibody
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with V5 tag antibody (GTX117997) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
V5 tag antibody detects V5 tag protein by immunofluorescent analysis.
Sample: V5-tag tagged protein transfected 293T cells were fixed in 4% PFA at RT for 15 min.
Green: V5 tag stained by V5 tag antibody (GTX117997) diluted at 1:500.
Blue: Hoechst 33342 staining.
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文献和实验Timblin GA et al., Mol Cell Biol 2017 (PMID:28373291)
Chang KH et al., J Formos Med Assoc 2016 (PMID:27423549)
Shimizu H et al., Sci Rep 2016 (PMID:27216889)
Lin PH et al., J Biomed Sci 2015 (PMID:26100518)
Liu YJ et al., FEBS Lett 2015 (PMID:25592834)
Kinoshita Y et al., Bone 2014 (PMID:25026495)
Lei L et al., PLoS One 2013 (PMID:23717625)
Veit M et al., Int J Mol Sci 2022 (PMID:36361998)
Yan X et al., Transl Oncol 2022 (PMID:35858494)
Yoann Abel et al., Nucleic Acids Res 2022 (PMID:35150569)
Jao TM et al., Cancer Lett 2021 (PMID:33271263)
Metje-Sprink J et al., Sci Rep 2020 (PMID:32737358)
Hoglin BE et al., Gen Comp Endocrinol 2020 (PMID:32213301)
Lin ST et al., Front Physiol 2019 (PMID:31681015)
Bernascone I et al., Nat Commun 2019 (PMID:31171792)
Wolverton EA et al., Gen Comp Endocrinol 2019 (PMID:31276671)
Chen KR et al., Viruses 2018 (PMID:30563052)
Barney E et al., Gen Comp Endocrinol 2019 (PMID:30468718)
Stephanie Grainger et al., bioRxiv 2018 (Epub)
Santhakumar D et al., Sci Rep 2018 (PMID:29717152)
Wang Z et al., Proc Natl Acad Sci U S A 2014 (PMID:24367099)
To engineer or manipulate antibody or Fv-based molecules, isolation of the V region of the antibodies is necessary. A number of strategies can be adopted for amplification; one such approach is to use subgroup-specific oligonucleotides
7.5, 150 mM NaCl, and1% (v/v) Triton X-100. 7. Suitable SDS-PAGE and transfer setup as in Section 2.4.1 . 8. Protein Sample Loading Dye (4×) as in Section 2.4.1 . 9. Nitrocellulose or PVDF membrane. 10. Mouse anti-β-catenin antibody (available
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
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