- ￥1700 - 4000
- GeneTex
- 美国
- GTX116557
- 2025年07月16日
- WB, ICC/IF, IHC-P
- Rabbit
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human DNA polymerase epsilon 3. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX116557
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P
- 浓度：
1.01 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
DNA polymerase epsilon 3
- 抗体英文名：
DNA polymerase epsilon 3 antibody [N1C3]
- 抗体名：
DNA polymerase epsilon 3 抗体 [N1C3]
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with DNA polymerase epsilon 3 antibody [N1C3] (GTX116557) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
DNA polymerase epsilon antibody [N1C3] detects DNA Polymerase epsilon protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
DNA Polymerase epsilon antibody [N1C3] (GTX116557) dilution: 1:1000.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using DNA polymerase epsilon(GTX116557) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunofluorescence analysis of PFA-fixed HeLa, using DNA polymerase epsilon(GTX116557) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate)
A: 293T
15% SDS PAGE
GTX116557 diluted at 1:1000
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文献和实验Zou W et al., J Virol 2017 (PMID:29237843)
In-Fusion Cloning with Vaccinia Virus DNA Polymerase
Vaccinia virus DNA polymerase (VVpol) encodes a 3′-to-5′ proofreading exonuclease that can degrade the ends of duplex DNA and expose single-stranded DNA tails. The reaction plays a critical role in promoting virus recombination in vivo
Production of Monoclonal Antibody by DNA Immunization with Electroporation
against a house dust mite allergen, designated as Blo t 11, which is a paramyosin homologue found in Blomia tropicalis mites. The Blo t 11 cDNA fused at the N terminus to the sequence of a signal peptide was cloned into the pCI mammalian expression vector. The DNA
Antibody Screening of Bacteriophage gt11 DNA Expression Libraries
from one species, it can be used to probe cDNA libraries from other species using DNA hybridization techniques. Alternatively, degenerate oligonucleotides can be designed from amino acid sequences from regions of proteins that are conserved across species
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