Recombinant protein encompassing a sequence within the center region of human CD73. The exact sequence is proprietary.
亚型:
IgG
形态:
Liquid
保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Polyclonal
标记物:
Unconjugated
适应物种:
Human, Mouse
保质期:
12 months from the shipping date of the product.
抗原来源:
Human
目录编号:
GTX113509
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Rabbit
应用范围:
WB, ICC/IF, IHC-P, Neutralizing/Inhibition, PLA
浓度:
0.28 mg/ml (Please refer to the vial label for the specific concentration.)
靶点:
CD73
抗体英文名:
CD73 antibody [N1N3]
抗体名:
CD73 抗体 [N1N3]
规格:
100 μl/25 μl
规格:
100 μl
产品价格:
¥4000.0
规格:
25 μl
产品价格:
¥1700.0
CD73 antibody [N1N3] detects CD73 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.Green: CD73 stained by CD73 antibody [N1N3] (GTX113509) diluted at 1:250.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [N1N3] (GTX113509) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CD73 antibody [N1N3] detects CD73 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CD73 antibody [N1N3] (GTX113509) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded J5 xenograft, using CD73(GTX113509) antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Sample (30 μg of whole cell lysate) A: HepG2 B: HCT116 12% SDS PAGE GTX113509 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [N1N3] (GTX113509) diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Application Reference
Aoki M et al., BMC Cancer 2019 (PMID:31510956)
Ma SR et al., Mol Cancer 2017 (PMID:28592285)
Nakao Y et al., Acta Biomater 2021 (PMID:33359765)
Casciaro F et al., Mech Ageing Dev 2020 (PMID:32800796)
M?ller GA et al., Mech Ageing Dev 2020 (PMID:32628941)