- ￥1700 - 4000
- GeneTex
- 美国
- GTX113303
- 2025年07月10日
- WB, ICC/IF, IHC-P, IHC-Wm, IP, ChIP assay
- Rabbit
- Human, Mouse, Rat, Zebrafish
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human HDAC3. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Zebrafish
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX113303
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Wm, IP, ChIP assay
- 浓度：
0.32 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
HDAC3
- 抗体英文名：
HDAC3 antibody
- 抗体名：
HDAC3 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
HDAC3 antibody immunoprecipitates HDAC3 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of HDAC3 protein by 2 μg of HDAC3 antibody (GTX113303) 7.5% SDS-PAGE The immunoprecipitated HDAC3 protein was detected by HDAC3 antibody (GTX113303) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody (GTX113303) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody (GTX113303) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using HDAC3(GTX113303) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody (GTX113303) diluted at 1:500.
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using HDAC3 antibody (GTX113303) at 1:300 dilution.
Immunohistochemical analysis (whole mount) of zebrafish embryo, using HDAC3 antibody (GTX113303) at 1:200 dilution.
HDAC3 antibody detects HDAC3 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody (GTX113303) diluted by 1:1000.
HDAC3 antibody detects HDAC3 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: HDAC3 protein stained by HDAC3 antibody (GTX113303) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody (GTX113303) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody (GTX113303) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or anti-HDAC3 antibody. The precipitated DNA was detected by PCR with primer set targeting to TFF1 or HK1.
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文献和实验Miscianinov V. et al., Molecular Therapy 2018 (Epub)
Armour SM et al., Nat Commun 2017 (PMID:28916805)
Emmett MJ et al., Nature 2017 (PMID:28614293)
Soriano FX et al., PLoS One 2011 (PMID:21695276)
Martirosian V et al., Cell Rep 2021 (PMID:34192534)
Miscianinov V et al., Mol Ther 2018 (PMID:29843955)
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