- ￥1700 - 4000
- GeneTex
- 美国
- GTX112794
- 2025年07月10日
- WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the N-terminus region of human Fibronectin. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX112794
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA
- 浓度：
0.34 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Fibronectin
- 抗体英文名：
Fibronectin antibody [N1N2], N-term
- 抗体名：
Fibronectin 抗体 [N1N2], N-term
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Fibronectin antibody [N1N2], N-term detects Fibronectin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
Fibronectin stained by Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Fibronectin antibody [N1N2], N-term detects Fibronectin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
Fibronectin stained by Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunofluorescence analysis of PFA-fixed HeLa, using Fibronectin (GTX112794) antibody at 1:200 dilution.
Fibronectin antibody [N1N2] detects Fibronectin protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2] (GTX112794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Indirect ELISA analysis was performed by coating plate with 100 μL of recombinant Fibronectin protein at concentration of 10 μg/mL. The coated protein is detected with Fibronectin antibody [N1N2], N-term (GTX112794) at rangeing from 0.5 to 140 ng/mL.
Competitive ELISA detection of human fibronectin in plasma using GTX112794. Recombinant or purified native Fibronectin protein was coated to an ELISA plate at concentration of 10 μg/ml. Human plasma was applied as the competitors and was serial diluted at 1, 20, 200, 2000, 20000, 200000X dilutions. The primary antibody concentration was diluted to 14 ng/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:2,000 and used to detect the primary antibody.
Fibronectin antibody [N1N2], N-term detects FN1 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
Fibronectin antibody [N1N2], N-term (GTX112794) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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