- ￥1700 - 4000
- GeneTex
- 美国
- GTX111664
- 2025年07月15日
- WB, ICC/IF, IHC-P, IHC-Wm
- Rabbit
- Human, Mouse, Rat, Zebrafish
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human IDE. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Zebrafish
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX111664
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Wm
- 浓度：
1.6 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
IDE
- 抗体英文名：
IDE antibody [N3C1], Internal
- 抗体名：
IDE 抗体 [N3C1], Internal
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Wild-type (WT) and IDE Kip1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE antibody [N3C1], Internal (GTX111664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE antibody [N3C1], Internal (GTX111664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
IDE antibody [N3C1], Internal detects Ide protein at the myotome on whole-mount zebrafish embryos by immunohistochemical analysis.?
Sample: PFA-fixed zebrafish embryos.
IDE antibody [N3C1], Internal (GTX111664) dilution: 1:200.
IDE antibody [N3C1], Internal detects IDE protein at cytosol on rat brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain stem.
IDE antibody [N3C1], Internal (GTX111664) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Various extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE antibody [N3C1], Internal (GTX111664) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
IDE antibody [N3C1], Internal detects IDE protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: IDE stained by IDE antibody [N3C1], Internal (GTX111664) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using insulin degrading enzyme(GTX111664) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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文献和实验Hamilton KA et al., PLoS One 2018 (PMID:29715265)
In the field of therapeutic recombinant proteins, monoclonal antibodies (mAbs) have achieved a rising success with more than 30 mAbs that have reached the market in the past 20 years. From a structural standpoint, one of the most important
. For most substrates, it is believed, though it has been demonstrated experimentally only for a few, that the first ubiquitin moiety is conjugated, via its C-terminal Gly76 residue, to an ε-NH2 group of an internal Lys residue. Recent findings indicate
Using MZEF to Find Internal Coding Exons
) was designed to help identify one of the most important classes of exons, i.e. the internal coding exons, in human genomic DNA sequences. It is neither for predicting intronless genes, nor for assembling predicted exons into complete gene models
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