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文献和实验High-Throughput Profiling of the Serum N-Glycome on Capillary Electrophoresis Microfluidics Systems
suitable samples for analysis. We here describe a short and robust assay to prepare 8-aminopyrene-1,3,6-trisulphonic acid-labeled N-glycans from serum samples. It only involves the subsequent addition of reagents and incubation
of the released oligosaccharides are then analyzed by capillary electrophoresis (CE) using a free solution separation in a bare fused silica capillary. An example of the application of the method to the analysis of the oligosaccharide population from antibodies
N-Glycosylation Analysis by HPAEC-PAD and Mass Spectrometry
among others. Here, we describe the profiling of N -glycans from glycoproteins of SKOV3 ovarian carcinoma cells by high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry
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