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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20
- 保质期：
1年
- 英文名：
Uracil Glycosylase Inhibitor (UGI)
- 库存：
999
- 供应商：
上海淳麦
- 规格：
1000U/10000U
The Uracil Glycosylase Inhibitor (UGI) of Bacillus subtilis bacteriophage PBS1 is a small protein (9.5 kDa) which inhibits E. coli uracil-DNA glycosylase (UDG) as well as UDG from other species. Inhibition of UDG occurs by reversible protein binding with a 1:1 UGD:UGI stoichiometry. UGI is capable of dissociating UDG-DNA complexes.
Product Source
An E. coli strain that carries the cloned UGI gene from Bacillus subtilis bacteriophage PBS1.
Reagents Supplied
The following reagents are supplied with this product:
| Store at (°C) | Concentration | |
| UDG Reaction Buffer | -20 | 10X |
Description
The Uracil Glycosylase Inhibitor (UGI) of Bacillus subtilis bacteriophage PBS1 is a small protein (9.5 kDa) which inhibits E. coli uracil-DNA glycosylase (UDG) as well as UDG from other species. Inhibition of UDG occurs by reversible protein binding with a 1:1 UGD:UGI stoichiometry. UGI is capable of dissociating UDG-DNA complexes.
Product Source
An E. coli strain that carries the cloned UGI gene from Bacillus subtilis bacteriophage PBS1.
Reagents Supplied
The following reagents are supplied with this product:
| Store at (°C) | Concentration | |
| UDG Reaction Buffer | -20 | 10X |
Advantages and Features
Applications
- Uracil glycosylase inhibitor (UGI) inhibits uracil-DNA glycosylase (UDG). Since UDG remains partially active following heat treatment at 95°C, UGI can be used to prevent subsequent degradation of product DNA.
Properties and Usage
Unit Definition
One unit of UGI is defined as the amount of protein required to inhibit one unit of E. coli UDG in 1 hour at 37°C in a total reaction volume of 50 µl. One unit of UDG is the amount of enzyme which will catalyze the release of 60 pmol of uracil per minute from double stranded, uracil-containing DNA.
Reaction Conditions
1X UDG Reaction Buffer
Incubate at 37°C
1X UDG Reaction Buffer:
20 mM Tris-HCl
1 mM DTT
1 mM EDTA
pH 8 @ 25°C
Storage Temperature
-20°C
Storage Conditions
10 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
200 μg/ml BSA
50% Glycerol
pH 7.4 @ 25°C
Heat Inactivation
No
Related Products
References
Lindahl, T. et al. (1977). J. Biol. Chem.. 252, 3286-3294.
Wang, Z., et al. (1991). Gene. 99, 31-37.
Bennett, S. E. and Mosbaugh, D. W. (1992). J. Biol. Chem.. 267, 22512-22521.
上海淳麦生物科技有限公司专注于感受态细胞、质粒、载体、农杆菌、酵母等生命科学领域的产品研发和技术服务支持。
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