- ¥3518
- Invitrogen™
- 4484453
- 美国
- 2025年10月31日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存：
10
- 英文名：
Total Exosome Isolation Reagent (from other body fluids)
- 供应商：
上海觅拓
- 保存条件：
Store at 2–8°C.
- 规格：
6ml
Description
Total Exosome Isolation Reagent (from other body fluids) enables fast and efficient enrichment of intact exosomes from cerebrospinal fluid (CSF), ascitic fluid, amniotic fluid, milk, and saliva with a protocol that is scalable depending on your sample size.
• Maximize recovery of intact exosomes for any type of downstream application
• Easily isolate exosomes using our simple and reliable protocol
• Avoid time-consuming ultra-centrifugation
• Maximize flexibility—can be scaled up or down depending on the sample size
By tying up water molecules, the Total Exosome Isolation Reagent forces less-soluble components such as vesicles out of solution, allowing them to be collected by a short, low-speed centrifugation. The reagent is added to the body fluid, and the solution is incubated at 2–8°C or room temperature (temperature and length of incubation depend on specific body fluid). The precipitated exosomes are recovered by standard centrifugation at 10,000 x g. The pellet is then resuspended in PBS or similar buffer, and the exosomes are ready for downstream analysis or further purification by affinity methods. Total RNA and protein can be purified using the Total Exosome RNA and Protein Isolation Kit (Cat# 4478545), or the intact exosomes can be used for biological studies of their pathways, functions, and trafficking.
For downstream mass spectrometry (MS) analysis we recommend performing gel electrophoresis purification of the isolated exosome suspension prior to analysis.
Specifications
| Reagent Type: | Exosome Isolation Reagent |
|---|---|
| Final Product: | Exosomes |
| Form: | Liquid |
| Product Size: | 6 mL |
Contents & storage
Store at 2–8°C.风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验的RNAiso Reagent量偏少。 ② 使用的组织材料中含有大量的有机溶剂（如：乙醇、异丙醇等)、高浓度的Buffer、碱性溶剂等。 ③ 如果提取的RNA中含有DNA时，可以使用DNase I (RNase Free；TaKaRa Code：D2215）进行DNA消化。 ■ 参考文献 1. J. Chirgwin et al, "Isolation of Biologically Active Ribonucleic Acid from Sources Enriched
Identification and Analysis of Circulating Exosomal microRNA in Human Body Fluids
, two types of RNA isolation methods are described with a focus on isolating RNA from body fluids, which can be more viscous than cell culture media.
. - Co-precipitant (optional): see DNA reagent (Amersham) or glycogen. - Fresh steril water (e.g. milliQ-purified) - Agarose gel (1%) containing ethidium bromide. Total RNA Isolation Protocol 1. Dissolve the tissue sample in buffer D
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