Matrices listed below were spiked with certain level of HB-EGF/HBEGF/Dtr/Dts/Hegfl/diphtheria toxin receptor (heparin-binding epidermal growth factor-like growthfactor) /DTRHEGFLdiphtheria toxin receptor (heparin-binding EGF-like growth factor) /DTS/DTSF/heparin-binding EGF-like growth factor/heparin-binding epidermal growth factor/proheparin-binding EGF-like growth factor and the recovery rates were calculated by comparing the measured value to the expected amount of HB-EGF/HBEGF/Dtr/Dts/Hegfl/diphtheria toxin receptor (heparin-binding epidermal growth factor-like growthfactor) /DTRHEGFLdiphtheria toxin receptor (heparin-binding EGF-like growth factor) /DTS/DTSF/heparin-binding EGF-like growth factor/heparin-binding epidermal growth factor/proheparin-binding EGF-like growth factor in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-102
93
EDTA plasma(n=5)
85-103
93
heparin plasma(n=5)
87-102
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HB-EGF/HBEGF/Dtr/Dts/Hegfl/diphtheria toxin receptor (heparin-binding epidermal growth factor-like growthfactor) /DTRHEGFLdiphtheria toxin receptor (heparin-binding EGF-like growth factor) /DTS/DTSF/heparin-binding EGF-like growth factor/heparin-binding epidermal growth factor/proheparin-binding EGF-like growth factor and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.