Matrices listed below were spiked with certain level of IL-9 (Interleukin 9) /IL9/HP40/P40/TCGFIII/TCGF3/P40 T-cell and Mast Cell Growth Factor/P40 Cytokine and the recovery rates were calculated by comparing the measured value to the expected amount of IL-9 (Interleukin 9) /IL9/HP40/P40/TCGFIII/TCGF3/P40 T-cell and Mast Cell Growth Factor/P40 Cytokine in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-100
94
EDTA plasma(n=5)
85-102
95
heparin plasma(n=5)
93-97
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-9 (Interleukin 9) /IL9/HP40/P40/TCGFIII/TCGF3/P40 T-cell and Mast Cell Growth Factor/P40 Cytokine and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.