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文献和实验of a master mix containing water, buffer, dNTPs, primers and Taq DNA Polymerase in a single tube, which can then be aliquoted into individual tubes. MgCl2 and template DNA solutions are then added. This method of setting reactions minimizes the possibility
This is a protocol from Engelke et.al.(1990)which was modified by Dr.Baron.The enzyme has been used for RT-PCR,genotyping and cloning.Solutions1000X IPTG0.5M IPTG 1.19g IPTGup to 10 ml wih sterile Qfilter and store at -20℃Buffer A50 mM Tris 7.9 25
Dideoxy Sequencing Reactions Using Taq Polymerase
where ambiguities are frequently found on the gels (see Fig.1 ). Currently several companies have developed sequencing kits using Tuq DNA polymerase. We have tried the TAQenceTM (U.S. Biochemicals, Cleveland, OH) and the Tuq multiwellTM (Amersham, Arlington Heights
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