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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified rat PDI protein.
- 亚型:
IgG2a
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat, Dog, Hamster, Pig, Rhesus Monkey, Primate
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Rat
- 目录编号:
GTX22792
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, IM, Neutralizing/Inhibition
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
PDI
- 抗体英文名:
PDI antibody [RL90]
- 抗体名:
PDI 抗体 [RL90]
- 规格:
100 μl
ICC/IF analysis of NIH-3T3 cells using GTX22792 PDI antibody [RL90].
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in PBS for 10 minutes at room temperature
Dilution : 1:75 for at least 1 hour at room temperature
WB analysis of 25 ug of HepG2 (Lane 1), Hela (Lane 2) and NIH-3T3 (Lane 3) cell lysates using GTX22792 PDI antibody [RL90].
Dilution : 1:1000
ICC/IF analysis of MDCK cells using GTX22792 PDI antibody [RL90].
Permeabilization : 0.1% Triton X-100
Dilution : 10 μg/ml in blocking buffer for at least 1 hour at room temperature
ICC/IF analysis of HMVEC Cells using GTX22792 PDI antibody [RL90].
Green : Primary antibody
Blue : Nuclei
ICC/IF analysis of U2OS cells using GTX22792 PDI antibody [RL90].
Red : Primary antibody
Green : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in PBS for 10 minutes at room temperature
Dilution : 1:75 for at least 1 hour at room temperature
ICC/IF analysis of NS-1 Cells using GTX22792 PDI antibody [RL90].
Green : Primary antibody
Blue : Nuclei
ICC/IF analysis of p19 Cells using GTX22792 PDI antibody [RL90].
Green : Primary antibody
Blue : Nuclei
IHC-P analysis of human lung adenocarcinoma tissue using GTX22792 PDI antibody [RL90].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200
IHC-P analysis of human colon tissue using GTX22792 PDI antibody [RL90].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200
FACS analysis of Hela cells using GTX22792 PDI antibody [RL90] compared to an isotype control (blue).
Dilution : 1 ug/test for 60 min at room temperature
Fixation : 2% PFA
IHC-P analysis of human tonsil tissue using GTX22792 PDI antibody [RL90].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200
FACS analysis of K562 cells using GTX22792 PDI antibody [RL90] compared to an isotype control (blue).
Dilution : 1 ug/test for 60 min at room temperature
Fixation : 2% PFA
ICC/IF analysis of A549 cells using GTX22792 PDI antibody [RL90].
Green : Primary antibody
Blue : Nuclei
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文献和实验Yating Cheng et al., Pakistan Journal of Zoology 2019 (Epub)
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