- ￥5300
- GeneTex
- 美国
- GTX22807
- 2025年07月13日
- WB, ICC/IF, IHC-P, IP, IHC
- Mouse
- Human, Mouse, Rat, Bovine, Chicken, Pig, Amphibians, Primate
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Purified bovine brain adaptor complexes.
- 亚型：
IgG2a
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Bovine, Chicken, Pig, Amphibians, Primate
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Bovine
- 目录编号：
GTX22807
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P, IP, IHC
- 浓度：
2 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody detects the products of both alpha-adaptin genes, alphaA and alphaC, as well as an alternatively spliced isoform of alphaA found in neurons.
- 抗体英文名：
alpha Adaptin antibody [AC1-M11]
- 抗体名：
alpha Adaptin 抗体 [AC1-M11]
- 规格：
500 μl
ICC/IF analysis of NRK cells using GTX22807 alpha Adaptin antibody [AC1-M11].
IHC-P analysis of human heart tissue using GTX22807 alpha Adaptin antibody [AC1-M11].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20
ICC/IF analysis of SH-SY5Y cells using GTX22807 alpha Adaptin antibody [AC1-M11]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% PFA
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature
WB analysis of tissue and membrane enriched extract (30 ug) of Rat Brain (Lane 1), SH-SY5Y (Lane 2), U-87 MG (Lane 3), COLO 205 (Lane 4), PC-3 (Lane 5), MCF7 (Lane 6), NIH/3T3 (Lane 7) and A-431 (Lane 8) using GTX22807 alpha Adaptin antibody [AC1-M11].
Dilution : 2 μg/ml
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文献和实验Ganapathy AS et al., Autophagy 2022 (PMID:34964704)
Murakami N et al., PLoS One 2012 (PMID:22514676)
In situ hybridisation to alpha satellite sequences (chromosome specific)
Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA
, upon immunization of H-2u mice with either myelin basic protein, or its immunodominant fragment, peptide Ac1-20, the Vβ8.2 TCR gene product is expressed in the majority of pathogenic T cells (2 –4 ). The restricted usage of the Vβ8.2 TCR gene product
marrow. The prime suspect: an adhesion molecule called alpha2 integrin. The molecule showed increased expression on memory cells, and its ligand is predominantly expressed in bone marrow tissue. Also, when the researchers blocked alpha2 integrin
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