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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
O-phospho-L-tyrosine and O-phospho-DL-tyramine
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Species independent
- 保质期:
12 months from the shipping date of the product.
- 目录编号:
GTX25592
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, FACS, IP, ELISA
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Phosphotyrosine
- 抗体英文名:
Phosphotyrosine antibody [IG2]
- 抗体名:
Phosphotyrosine 抗体 [IG2]
- 规格:
50 μg
Tyrosine phosphorylated c-Abl and Bcr-Abl was performed following immunoprecipitation of K562 cell lysates using a c-Abl antibody, from untreated cells (left lane) or cells treated with 10uM of the tyrosine kinase inhibitor Gleevec (right lane) for 24 hours. WB was performed using GTX25592 Phosphotyrosine antibody [IG2] at a dilution of 1:250.
IP analysis of K562 cells (left lane) or cells treated with 10uM of the tyrosine kinase inhibitor Gleevec (right lane) for 24 hours using GTX25592 Phosphotyrosine antibody [IG2].
IP antibody:GTX25592
IB antibody : c-abl antibody
IP reaction : 2μg antibody / 500μg lysate
ICC/IF analysis of HeLa cells using GTX25592 Phosphotyrosine antibody [IG2].
Green : Primary antibody
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文献和实验Enrichment of Phosphotyrosine Proteome of Human Platelets by Immunoprecipitation
Proteomics offers the opportunity to comprehensively investigate the anucleate platelet. Here, we present a detailed procedure for enrichment by immunoprecipitation, using the monoclonal antibody 4G10, of the dynamic phosphotyrosine proteome
有anti-phosphothronine 和anti-phosphotyrosine的抗体,PAb和MAb都有,但是没有anti-phosphoserine的抗体。 摘抄: Anti-Phosphothreonine (MAb) - CryptateThe anti-phosphothreonine antibody is a monoclonal antibody (clone 42H4) produced at Cell Signaling
Kamps's Western Blotting Protocol
in immunoprecipitates than anti-immunoglobulin antibodies.ECL analysis of immunoprecipitates using a second antibody is almost always blighted by strong staining of the antibody bands and by the staining of abundant proteins in the sample.Blots developed with iodinated
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