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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Intercellular membrane protein-containing vesicles (containing GLUT4) from rat.
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Rat, Hamster
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Rat
- 目录编号:
GTX79350
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, IHC-P, FACS, IP
- 靶点:
Caveolin 1
- 抗体英文名:
Caveolin 1 antibody [7C8]
- 抗体名:
Caveolin 1 抗体 [7C8]
- 规格:
100 μl
FACS analysis of CHO cells using GTX79350 Caveolin 1 antibody [7C8] compared to an isotype control (blue).
Dilution : 1:20 for 60 min at room temperature
Fixation : 2% PFA
ICC/IF analysis of H-4-II-E Cells using GTX79350 Caveolin 1 antibody [7C8]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC
FACS analysis of H-4-II-E cells using GTX79350 Caveolin 1 antibody [7C8] compared to an isotype control (blue).
Dilution : 1:20 for 60 min at room temperature
Fixation : 2% PFA
IHC-P analysis of rat lung tissue using GTX79350 Caveolin 1 antibody [7C8].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20
WB analysis of 25 ug of A431 (Lane 1) and rat lung (Lane 2) cell lysates using GTX79350 Caveolin 1 antibody [7C8].
Dilution : 1:1000
IHC-P analysis of rat lymph node tissue using GTX79350 Caveolin 1 antibody [7C8].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20
ICC/IF analysis of HeLa cells using GTX79350 Caveolin 1 antibody [7C8]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : 4% PFA
Permeabilization : 0.1% Triton X-100 in PBS for 15 minutes
Dilution : 1:100 for 1 hour at room temperature
ICC/IF analysis of cells using GTX79350 Caveolin 1 antibody [7C8].
FACS analysis of L6 cells using GTX79350 Caveolin 1 antibody [7C8] compared to an isotype control (blue).
Dilution : 1:20 for 60 min at room temperature
Fixation : 2% PFA
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文献和实验Ise H et al., Genes Cells 2017 (PMID:28898551)
Liu IF et al., Biol Direct 2023 (PMID:36879344)
祝贺〖雅裕睿安〗成为Signalway Antibody(SAB)独家
祝贺〖上海雅裕 〗成为 Signalway Antibody 品牌 (SAB) 的独家总代理。 促销【 ABCD 】细则如下: 【 A 】 买两支 SAB 抗体免费获得 8G 优盘 活动时间:即日起,至 2011 年
Mapping of Protein Transduction Pathways with Fluorescent Microscopy
toxin (ambiguous marker for clathrin-, caveolin-, and flotillin-mediated, but also clathrin- and caveolin-independent endocytosis) to determine the role of the respective pathways during entry to cells, and to different intracellular targets
Detergent and Detergent-Free Methods to Define Lipid Rafts and Caveolae
that isolate buoyant lipid rafts and caveolae as well as caveolin antibody-based immunoisolation of detergent-insoluble membranes that selectively isolates caveolae and not lipid rafts. Also, a general method to disrupt lipid rafts and caveolae using β
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