- ￥5600
- GeneTex
- 美国
- GTX80693
- 2025年07月14日
- WB, ICC/IF, FACS, IP, Dot, ChIP assay
- Mouse
- Species independent
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthetic peptide sequence surrounding the acetylated lysine 9 of histone H3.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Species independent
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Mouse
- 目录编号：
GTX80693
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, FACS, IP, Dot, ChIP assay
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Acetyl Lysine
- 抗体英文名：
Acetyl Lysine antibody [1C6]
- 抗体名：
Acetyl Lysine 抗体 [1C6]
- 规格：
100 μg
ChIP analysis of 4x10⁶ LNCaP cells using GTX80693 Acetyl Lysine antibody [1C6]. Quantitation of immunoprecipitated GAPDH promoter sequence is presented as fold enrichment of the acetyl lysine monoclonal antibody versus non-specific IgG.
ICC/IF analysis of HeLa cells untreated (left panels) or treated with 0.3uM Trichostatin A for 16 hours (right panels) using GTX80693 Acetyl Lysine antibody [1C6].
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
WB analysis of untreated (DMSO only) or cells treated with 0.3uM or 3uM of Trichostatin A (TSA) for 16 hours using GTX80693 Acetyl Lysine antibody [1C6].
Dilution : 1:1000
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文献和实验Hung YH et al., Am J Cancer Res 2022 (PMID:35261797)
Wu AC et al., J Exp Clin Cancer Res 2022 (PMID:35109908)
Wu CC et al., Redox Biol 2018 (PMID:30121389)
Protocols for Lysine Conjugation
Currently, the most widely used chemical methodology for the conjugation of drugs to monoclonal antibodies involves either lysine or cysteine residues. In this chapter, several methods for the preparation of antibody–drug conjugates (ADCs
How Do I make an Antibody against a Peptides
-Activated Carrier Proteins (Figure 1). If your hapten does not have a sulfhydryl group, you can use EDC chemistry (Figure 2) to couple amines (lysine) or carboxylic acids (glutamic, aspartic acid or 5'-phosphate) to the carrier protein. Keyhole limpet
make an Antibody against a Peptides
make an Antibody against a Peptides If your peptide is synthesized with a terminal cysteine residue (i.e., a free sulfhydryl group, -SH), you can use any maleimide-activated carrier protein
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