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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
The antiserum was produced against synthesized phosphopeptide derived from human p44/42 MAP Kinase around the phosphorylation site of tyrosine 204 (T-E-Yp-V-A).
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX78987
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, IHC-P
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
ERK1 (phospho Tyr204) + ERK2 (phospho Tyr187)
- 抗体英文名:
ERK1 (phospho Tyr204) + ERK2 (phospho Tyr187) antibody
- 抗体名:
ERK1 (phospho Tyr204) + ERK2 (phospho Tyr187) 抗体
- 规格:
100 μg
IHC-P analysis of human breast carcinoma tissue using GTX78987 ERK1 (phospho Tyr204) + ERK2 (phospho Tyr187) antibody.
WB analysis of NIH-3T3 cell lysate using GTX78987 ERK1 (phospho Tyr204) + ERK2 (phospho Tyr187) antibody (Lane 3 and 4) and ERK1/2 antibody (Lane 1 and 2).
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文献和实验Localization and Trafficking of Fluorescently Tagged ERK1 and ERK2
The action of ERK1 and ERK2 activity on the nuclear substrates requires crossing the nuclear envelope and the localization of phospho-ERK into the nucleus. The nucleo-cytoplasmic trafficking of ERK is therefore crucial for the correct
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
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