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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of Dengue virus NS3 protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Dengue virus
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Dengue virus
- 目录编号:
GTX124252
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P, FACS, IP, IHC
- 浓度:
0.15 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Dengue virus NS3 protein
- 抗体英文名:
Dengue virus NS3 protein antibody
- 抗体名:
Dengue virus NS3 protein 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Non-infected (–) and infected (+) BHK-21 whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus NS3 protein antibody (GTX124252) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus NS3 protein antibody (GTX124252) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
Blue: Hoechst 33342 staining.
NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Sample (20 μg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: JEV infect BHK-21
7.5% SDS PAGE
GTX124252 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
Blue: Hoechst 33342 staining.
NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein by western blot analysis.
A. 30 μg BHK-21 whole cell extract
B. 30 μg whole cell extract of Dengue virus type 1 infected BHK-21 cells
C. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
D. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
E. 30 μg whole cell extract of Dengue virus type 4 infected BHK-21 cells
7.5% SDS-PAGE
NS3 (Dengue virus) antibody (GTX124252) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
Blue: Hoechst 33342 staining.
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against dengue, timely diagnosis is therefore necessary for patient management. Laboratory diagnosis is carried out by virus isolation, demonstration of viral antigen, presence of viral nucleic acid, and antibodies. Further, recombinant dengue envelope protein
Fluorimetric and HPLC-Based Dengue Virus Protease Assays Using a FRET Substrate
The number of dengue virus infections is increasing and the dengue NS2B–NS3 protease is considered a promising target for the development of antiviral therapies. Therefore, reliable and fast screening systems are needed for the discovery
Characterization of the Dengue Virus Envelope Glycoprotein Expressed in Pichia pastoris
The full-length and truncated forms of recombinant envelope (E) glycoprotein from Dengue virus type 1, Singapore strain S275/90 were expressed in the yeast, Pichia pastoris , using a secretory vector. A truncated form of the E protein
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