- ￥4000
- GeneTex
- 美国
- GTX124207
- 2026年02月26日
- WB, ICC/IF, IHC-P, IHC-Wm, IP, ChIP assay, IHC
- Rabbit
- Human, Mouse, Rat, Monkey
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human TET1. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Monkey
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX124207
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Wm, IP, ChIP assay, IHC
- 浓度：
1.05 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
TET1
- 抗体英文名：
TET1 antibody [N3C1]
- 抗体名：
TET1 抗体 [N3C1]
- 规格：
100 μl
Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (GTX124207) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (GTX124207) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
TET1 antibody [N3C1] detects TET1 protein at nucleus by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% PFA at RT for 15 min.
Green: TET1 stained by TET1 antibody [N3C1] (GTX124207) diluted at 1:1000.
Blue: Hoechst 33342 staining.
HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (GTX124207) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded Hela xenograft, using TET1 (GTX124207) antibody at 1:1000 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
TET1 antibody [N3C1] detects TET1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: TET1 stained by TET1 antibody [N3C1] (GTX124207) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
TET1 antibody [N3C1] detects TET1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of DDDDK-human TET1-transfected 293T cells
5% SDS-PAGE
TET1 antibody [N3C1] (GTX124207) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
TET1 antibody [N3C1] detects TET1 protein at nucleus on Human normal prostate tissue by immunohistochemical analysis.
Sample: Paraffin-embedded Human normal prostate tissue.
TET1 antibody [N3C1] (GTX124207) dilution: 1:1000.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
TET1 antibody [N3C1] detects TET1 protein at nucleus in human A549 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded human A549 xenograft .
TET1 antibody [N3C1] (GTX124207) diluted at 1:250.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
TET1 antibody [N3C1] detects TET1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
TET1 stained by TET1 antibody [N3C1] (GTX124207) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献和实验Application Reference
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In the field of therapeutic recombinant proteins, monoclonal antibodies (mAbs) have achieved a rising success with more than 30 mAbs that have reached the market in the past 20 years. From a structural standpoint, one of the most important
Materials 0.1M NaHC03 pH9 DMSO NHS-Biotin (N-Hydroxysuccinimidobiotin, Sigma #H-1759) PBS Procedure Dialyze the sample against carbonate buffer. After dialysis, adjust
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
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