- ￥5300
- GeneTex
- 美国
- GTX44344
- 2025年07月10日
- Mouse
- Species independent
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原：
Biotin-KLH
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Species independent
- 保质期：
12 months from the shipping date of the product.
- 目录编号：
GTX44344
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 浓度：
8.85 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Recognizes free biotin, biotin conjugated to KLH or HRP, and biotinylated DNA probes.
- 抗体英文名：
Biotin antibody [B169M]
- 抗体名：
Biotin 抗体 [B169M]
- 规格：
1 mg
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- 作者
- 内容
- 询问日期
文献和实验The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
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