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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human MAD1. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX105079
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P, IP
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
MAD1
- 抗体英文名:
MAD1 antibody
- 抗体名:
MAD1 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Immunoprecipitation of MAD1 protein from HeLa whole cell extracts using 5 μg of MAD1 antibody (GTX105079).
Western blot analysis was performed using MAD1 antibody (GTX105079).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Immunofluorescence analysis of PFA-fixed MCF-7, using MAD1(GTX105079) antibody at 1:500 dilution.
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using MAD1(GTX105079) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
MAD1 antibody detects MAD1L1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
E. 30 μg A375 whole cell lysate/extract
7.5% SDS-PAGE
MAD1 antibody (GTX105079) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献和实验Sobajima T et al., J Cell Biol 2023 (PMID:36897279)
Chen L et al., Cell Prolif 2023 (PMID:36354207)
Zo? Geraghty et al., J Cell Sci 2021 (PMID:33288550)
Chen Q et al., Cell Rep 2021 (PMID:34551298)
Hayward D et al., J Cell Biol 2019 (PMID:31511308)
Daniel Hayward et al., bioRxiv 2019 (Epub)
Hayward D et al., J Cell Biol 2019 (PMID:30674582)
Alfonso-P?rez T et al., J Cell Biol 2019 (PMID:30674583)
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
General comments: Antibodies, like most proteins, do not like to be freeze-thawed. Avoid repetitive freezing of your solution. The best way to store your antibody is to keep a high protein concentration (>1 mg/ml), add some protease
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