- ￥390
- 钦诚生物
- 上海
- QC25-03645
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
室温
- 保质期：
一年
- 供应商：
钦诚生物
- 规格：
250ML
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文献和实验solution 3. Incubate with secondary antibody diluted in Blocking buffer for 60 min at room temp. 4. 3 x10min secondary washing solution 1 x10min alkaline phosphatese buffer 5. Detect with Chromogenic I. Stripping blot 1. Rinse blot off with 0.05
WHOLE MOUNT IN SITU HYBRIDIZATION. Mouse Embryos
Use gloves and aerosol barrier tips for changing the solutions from the fixation step to the end of hybridization. Preparation of embryos Dissect embryos in cold PBS , change solution often. Punch a hole
genomic DNA elute/ modified lysis buffer solution (15 minutes at R/T under constant motion);5.Following the 15 minute incubation the beads are captured using a magnetic bead separator (Invitrogen™) and washed 3 times with 2x wash buffer
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