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室温
- 保质期:
一年
- 供应商:
钦诚生物
- 规格:
250ML
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文献和实验SDS-PAGE Western Blotting Protocols
is the same as the that of the spacers’. 6. Remove comb. 7. Pour resolving gel to the mark in step 5. Optional: Create an agarose plug with newly liquified 1% agarose (i.e., the agarose needs to be hot when used). The agarose does not affect the running of the SDS
Preparing and Running a Protein Gel (7% Polyacrylamide)
实验步骤 1. Preparation of Running Gel Solution 1) Add to a 50 ml cylinder: DD-H2 O 26 ml 30% acrylamide stock (37.5:1 Acryl/Bis) 10.5 ml 2M Tris 8.8 8.4 ml 20% SDS 0.23 ml 2) Cover
8.8 (resolving gel) 1.0 M Tris, pH 6.8 (stacking gel) 5x SDS Running Buffer (1 L) Tris 15 g Glycine 72 g SDS 5 g Coomassie Blue Stain 10% (v/v) acetic acid 0.006% (w/v) Coomassie Blue dye 90% ddH2O Isopropanol Fixing Solution 10
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