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- 钦诚生物
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- QC25-00660
- 2025年07月07日
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文献和实验Transepithelial Measurements of Bicarbonate Secretion in Calu-3 Cells
The importance of impaired HCO 3 secretion in the pathophysiology of the pancreas of cystic fibrosis (CF) patients has been well documented and known for many years ( 1 ). Studies in 1990s from the Welsh ( 2 ) and Boucher ( 3 ) laboratories
How to Measure CFTR-Dependent Bicarbonate Transport: From Single Channels to the Intact Epithelium
Bicarbonate serves many functions in our body. It is the predominant buffer maintaining a physiological pH in the blood and within our cells. It is also essential for proper digestion of nutrients and solubilization of complex protein
Cl(pH7.6) 20mM Tween-20 1‰(v/v) ⑤TBS NaCl 150mM Tris・Cl(pH7.6) 20mM ⑥溶液A � 0.1M Tris・Cl(pH9.0) 将3.026g Tris Base溶解于200mL双蒸水后调pH至9.0，双蒸水定容至250mL。 � 0.4mM PCA 将4.45mg PCA溶解于4mL 0.1M Tris・Cl(pH9.0)中。 � 100g/L luminol 将75mg luminol溶解
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