- ￥10600
- GeneTex
- 美国
- GTX42244
- 2025年07月12日
- FACS, Neutralizing/Inhibition
- Rat
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Murine LAG3 Ig fusion protein.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Mouse
- 目录编号：
GTX42244
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rat
- 应用范围：
FACS, Neutralizing/Inhibition
- 浓度：
1.0 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody recognizes an epitope within the D2 domain of LAG3.
- 抗体英文名：
LAG3 antibody [C9B7W] (Low endotoxin, azide free)
- 抗体名：
LAG3 抗体 [C9B7W] (Low endotoxin, azide free)
- 规格：
500 μg
FACS analysis of CD223 transfected cells using GTX42244 LAG3 antibody [C9B7W] (Low endotoxin, azide free).
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文献和实验Scarlett UK et al., J Exp Med 2012 (PMID:22351930)
Meng Q et al., Invest Ophthalmol Vis Sci 2006 (PMID:17003438)
Purification of Endotoxin Free mAbs
pressure of circulation to force water out through the membrane, and rapidly concentrating the antibody/proteins up to 100 fold. The advantage of this method is very large volumes can be processed using sterile and endotoxin free components. Ammonium
Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
Phycoerythrin conjugation protocol
and Centricon concentrators. Add 0.5% sodium azide. Store at 4 °C protected from light. Do not freeze. Figure 1. Analysis of column eluate fractions from PE conjugation of monoclonal antibody IB4 (murine IgG2a, anti-human CD
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