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对话丨深圳先进技术研究院赵乔团队的「可编程植物」探索abinScience 体内级抗体,年度锁价,低至 1200五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Rat spleen cells
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Rat, Bovine
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Rat
- 目录编号:
GTX41868
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, RIA
- 浓度:
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
CD68
- 抗体英文名:
CD68 antibody [ED1]
- 抗体名:
CD68 抗体 [ED1]
- 规格:
100 μg
IHC analysis of rat liver, with induced hepatocellular damage tissue using GTX41868 CD68 antibody [ED1].
IHC-Fr analysis of rat lymph node tissue using GTX41868 CD68 antibody (red n A) and Mouse anti Rat CD4 (Green in B). C is the merged image with nuclei counter-stained blue using DAPI. Low power.
IHC-Fr analysis of rat lymph node tissue using GTX41868 CD68 antibody [ED1].
IHC-Fr analysis of rat lymph node tissue using GTX41868 CD68 antibody [ED1].
IHC-Fr analysis of rat lymph node tissue using GTX41868 CD68 antibody (red n A) and Mouse anti Rat CD4 (Green in B). C is the merged image with nuclei counter-stained blue using DAPI.Medium power
IHC-Fr analysis of rat lymph node tissue using GTX41868 CD68 antibody (red n A) and Mouse anti Rat CD4 (Green in B). C is the merged image with nuclei counter-stained blue using DAPI. High power.
IHC-Fr analysis of rat lymph node tissue using GTX41868 CD68 antibody [ED1].
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文献和实验Mang Y et al., Ann Transl Med 2022 (PMID:36330418)
Hung HS et al., Cells 2021 (PMID:34831077)
Tseng WC et al., Stem Cell Res Ther 2021 (PMID:34183058)
He S et al., Inflammation 2021 (PMID:33738649)
Kao YJ et al., AJNR Am J Neuroradiol 2019 (PMID:30923084)
Mahara A et al., J Biomed Mater Res A 2020 (PMID:32323458)
Hou HH et al., Am J Physiol Lung Cell Mol Physiol 2018 (PMID:29722565)
Mukwaya A et al., Angiogenesis 2018 (PMID:29445990)
Jeuthe S et al., J Cardiovasc Magn Reson 2016 (PMID:27084492)
Tsutsumi K et al., JPS 2016 (Epub)
Schmerler P et al., Acta Physiol (Oxf) 2014 (PMID:24410878)
Murata K et al., Int J Mol Med 2022 (PMID:35686541)
大鼠ED-1/sCD68分子(ED-1)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠 ED-1/sCD68 分子 (ED-1)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 CD68 单抗包被于酶标板上,标准品和样品中的 CD68 与单抗结合,加入生物素化的抗大鼠 CD68 ,形成免疫
Elabscience|巨噬细胞极化总失败?RAW264.7/THP-1/BMDM/hMDM四大模型避坑指南与实操全攻略
16/32 Antibody,轻轻吹打混匀,室温避光孵育10 min封闭细胞表面的Fc受体; (4)加入各流式抗体(5 μL/test),轻轻吹打混匀,4℃避光孵育30 min; (5)孵育完成后,每管加入1 mL Cell Staining Buffer轻轻吹打混匀,150 × g离心3 min,弃上清; (6)加入200 μL Cell Staining Buffer重悬细胞沉淀,上机检测。 图1. Raw264.7细胞极化培养流程 2. Raw264.7细胞向M1型巨噬细胞极化结果 图
Elabscience 巨噬细胞三大功能及检测方案全梳理,看完少走90%弯路!
和IF是组织切片中检测巨噬细胞的最经典方法。其原理是用特异性抗体识别巨噬细胞标志物,再通过酶促显色(如DAB呈棕色)或荧光标记实现可视化。人类组织最常用的巨噬细胞标志物是CD68(泛巨噬细胞标志物)。但单一标志物无法识别全部亚群:例如小鼠中F4/80标记成熟组织驻留巨噬细胞,却存在F4/80阴性、CD68阳性的细胞;CD11b常被视为单核来源巨噬细胞的标志,但在中性粒细胞也呈阳性。 2. 流式细胞术(Flow Cytometry) 流式细胞术是目前应用最广泛的巨噬细胞定量检测方法之一,尤其适用于单细胞
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