- ￥1700 - 4000
- GeneTex
- 美国
- GTX103031
- 2025年07月12日
- WB, ICC/IF, IHC-P
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the C-terminus region of human SP1. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX103031
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
SP1
- 抗体英文名：
SP1 antibody
- 抗体名：
SP1 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
SP1 antibody detects SP1 protein at nucleus by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% PFA at RT for 15 min.
Green: SP1 protein stained by SP1 antibody (GTX103031) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 6 μm.
Sample (30 ug of whole cell lysate)
A: THP-1
5% SDS PAGE
GTX103031 diluted at 1:1000
SP1 antibody detects SP1 protein at nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
SP1 antibody (GTX103031) diluted at 1:250.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
SP1 antibody detects SP1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody (GTX103031) diluted by 1:500.
SP1 antibody detects SP1 protein at nucleus on human gastric carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human gastric carcinoma.
SP1 antibody (GTX103031) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
SP1 antibody detects SP1 protein at nucleus on human gastric carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human gastric carcinoma.
SP1 antibody (GTX103031) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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文献和实验wuyf 我想看转录因子sp1对我的目的基因的表达是不是有影响，我用sp1siRNA转染细胞，发现与对照细胞相比，细胞内总RNA量减少。sp1siRNA转染细胞大家有做过的吗？ freecell 同样结果重复了几次么？做了几个剂量？每个剂量做了几个重复？ ffyy8888 :D因为转染了一些东西进去，或多或少会对细胞状态有影响的，你只要将对照做全，就可以呀，怕啥，多做几组对照
An Efficient Way of Studying ProteinProtein Interactions Involving HIF-, c-Myc, and Sp1
of mechanisms of cellular processes in biology and diseases. The hypoxia-inducible transcription factor HIF-1α possesses a non-transcriptional activity that competes with c-Myc for Sp1 binding, whereas its isoform HIF-2α lacks Sp1-binding activity
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
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