Matrices listed below were spiked with certain level of PKR1/GPR73/GPR73a/PROKR1/ZAQ/G protein-coupled receptor 73/G protein-coupled receptor ZAQ/GPR73/GPR73aG-protein coupled receptor 73/PK-R1/PKR1G-protein coupled receptor ZAQ/prokineticin receptor 1 and the recovery rates were calculated by comparing the measured value to the expected amount of PKR1/GPR73/GPR73a/PROKR1/ZAQ/G protein-coupled receptor 73/G protein-coupled receptor ZAQ/GPR73/GPR73aG-protein coupled receptor 73/PK-R1/PKR1G-protein coupled receptor ZAQ/prokineticin receptor 1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-105
93
EDTA plasma(n=5)
91-104
97
heparin plasma(n=5)
85-96
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PKR1/GPR73/GPR73a/PROKR1/ZAQ/G protein-coupled receptor 73/G protein-coupled receptor ZAQ/GPR73/GPR73aG-protein coupled receptor 73/PK-R1/PKR1G-protein coupled receptor ZAQ/prokineticin receptor 1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.