- ￥1700 - 4000
- GeneTex
- 美国
- GTX100454
- 2025年07月11日
- WB, IHC-P, IHC-Fr
- Rabbit
- Human, Mouse, Rat
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human IGFBP3. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX100454
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, IHC-P, IHC-Fr
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
IGFBP3
- 抗体英文名：
IGFBP3 antibody [N2C3]
- 抗体名：
IGFBP3 抗体 [N2C3]
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
IGFBP3 antibody [N2C3] detects IGFBP3 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: IGFBP3 protein stained by IGFBP3 antibody [N2C3] (GTX100454) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
U87-MG whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IGFBP3 antibody [N2C3] (GTX100454) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Mouse plasma (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with IGFBP3 antibody (GTX100454) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
IGFBP3 antibody [N2C3] detects secreted IGFBP3 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
IGFBP3 stained by IGFBP3 antibody [N2C3] (GTX100454) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Johnson TK et al., Am J Physiol Heart Circ Physiol 2019 (PMID:31418583)
Wang HH et al., Tumour Biol 2017 (PMID:28378642)
Shih HJ et al., Sci Rep 2021 (PMID:34824343)
Shih HJ et al., Am J Cancer Res 2020 (PMID:32642286)
In the field of therapeutic recombinant proteins, monoclonal antibodies (mAbs) have achieved a rising success with more than 30 mAbs that have reached the market in the past 20 years. From a structural standpoint, one of the most important
Materials 0.1M NaHC03 pH9 DMSO NHS-Biotin (N-Hydroxysuccinimidobiotin, Sigma #H-1759) PBS Procedure Dialyze the sample against carbonate buffer. After dialysis, adjust
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
技术资料暂无技术资料 索取技术资料
