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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to aa 2-32 of Human MIF conjugated to keyhole limpet hemocyanin (KLH). MIF is a proinflammatory cytokine that plays an i
- 亚型:
IgY
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX48478
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Chicken
- 应用范围:
WB, ELISA
- 浓度:
5 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
MIF
- 抗体英文名:
MIF antibody
- 抗体名:
MIF 抗体
- 规格:
100 μg
Western blot analysis of GeneTex's IgY fraction of Chicken-anti-Human MIF polyclonal antibody shows the detection of 100 μg of recombinant MIF present in a lysate. Similar detection of MIF will occur when human serum is analyzed. In lane 1 no reaction is observed in the control whereas lane 2 shows a single band at 12.3 kDa. A 4-20% gradient gel was used to separate the proteins by SDS-PAGE. The protein was transferred to nitro-cellulose using standard methods. After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye800 conjugated Gt-a-Chicken Rabbit IgG [H&L] for 1 h at room temperature. LICOR's OdysseyR Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
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文献和实验基于多重免疫荧光 (mIF) 技术的空间表型特征用以开发新一代生物标志物
越来越多的研究表明,基于多重免疫荧光(mIF)技术的空间表型特征正在用以开发新一代生物标志物,它使用多重抗体面板在完整组织样本中通过所处环境中的功能和状态来描述肿瘤和免疫细胞的特征。不同的细胞密度模式和相互作用构成的细胞间肿瘤微环境中(TME)已被证明可以对疾病结果和免疫治疗反应高度预测。 在《空间表型特征:表征实体肿瘤和预测免疫治疗反应的新型生物标志物》白皮书中介绍了基于 mIF 的空间表型特征作为新一代生物标志物发现的案例,意义和价值。 目前,只有少数经 FDA 批准
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
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