- ￥5300
- GeneTex
- 美国
- GTX48653
- 2025年07月09日
- WB, ELISA, IHC
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-32A protein.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Biotin
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX48653
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ELISA, IHC
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-32A in cell supernatants and certain body fluids.
- 抗体英文名：
IL32A antibody (Biotin)
- 抗体名：
IL32A 抗体 (Biotin)
- 规格：
50 μg
Western blot using GeneTex's HRP conjugated anti-Human IL-32A antibody shows detection of a band ~19 kDa in size corresponding to recombinant human IL-32A. The identity of the higher molecular weight band is unknown. Molecular weight markers are shown (left). After transfer, the membrane was blocked with 3% BSA in TBS followed by reaction with antibody at a 1:5,000 dilution for 30 min at room temperature. Detection occurred using TMB substrate.
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文献和实验The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
sample to 1 mg/ml (or less). Prepare 1 mg/ml solution of NHS-Biotin in DMSO. Add NHS-Biotin in DMSO to antibody at desired ratio e.g. for 1:8 ratio add 125 ml of NHS-Biotin to 1mg of Ab Incubate on rotator for 4 hours at room
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