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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids at the carboxyl terminus of the Cyclin E2 protein.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX48724
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, IP, ELISA
- 浓度:
1.17 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Cyclin E2
- 抗体英文名:
Cyclin E2 antibody
- 抗体名:
Cyclin E2 抗体
- 规格:
50 μg
Western blot using GeneTex's affinity purified anti-Cyclin E2 antibody shows specific detection of Cyclin E2. Cell extracts over-expressing mouse Cyclin E1 (lane 1) and Cyclin E2 (lane 2) were electrophoresed, transferred to nitrocellulose, and probed with the anti-Cyclin E2 antibody. The affinity purified antibody also detects endogenous Cyclin E2 in Skp2-/- MEF cells. (data not shown).
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文献和实验Zhang G et al., Eur J Pharmacol 2020 (PMID:32950500)
Assays for Cyclin-Dependent Kinase Inhibitors
of the Retinoblastoma gene product (Rb) by CDK4 and/or CDK6 (G1/S checkpoint), leading to the release of bound E2F from Rb and allowing the transcription of genes by “free E2F” necessary for S phase progression. During late G1 phase, CDK2/cyclin E complex phosphorylates
Immunodetection of cyclin D1 and D2/D3 using flow cytometry
that has been kept -20°C. Incubate the cells for at least two hours. Wash once with staining buffer and decant. Add the primary anti-cyclin antibody in a volume of 200 µ l. Incubate overnight at 4°C. Wash twice with cold cyclin staining buffer and add the Cy
E2F Transcription Factors and pRb Pocket Proteins in Cell Cycle Progression
The E2F-family of transcripion factors exerts fascinating and contrasting functions in tran-scriptional repression and activation of genes regulating proliferation, apoptosis, and differentiation. E2F is principally regulated by its temporal
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