- ￥7300
- GeneTex
- 美国
- GTX83426
- 2025年07月16日
- WB, ICC/IF, IHC-P
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Human recombinant protein fragment corresponding to amino acids 27-233 of human VEGFA (NP_003367) produced in E.coli.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX83426
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
VEGFA
- 抗体英文名：
VEGFA antibody [4E3]
- 抗体名：
VEGFA 抗体 [4E3]
- 规格：
100 μl
WB analysis of various cell lines using GTX83426 VEGF antibody [4E3].
Loading : 10 ug per lane
Dilution : 1:200
IHC-P analysis of human pancreas tissue using GTX83426 VEGF antibody [4E3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
ICC/IF analysis of COS7 cells transiently transfected with VEGF plasmid using GTX83426 VEGF antibody [4E3].
IHC-P analysis of human breast tissue using GTX83426 VEGF antibody [4E3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
WB analysis of HEK293T cells transfected with VEGF plasmid (Right) or empty vector (Left) for 48 hrs using GTX83426 VEGF antibody [4E3].
Loading : 5 ug per lane
IHC-P analysis of human breast adenocarcinoma tissue using GTX83426 VEGF antibody [4E3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
IHC-P analysis of human liver tissue using GTX83426 VEGF antibody [4E3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
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文献和实验De Francesco F et al., J Int Med Res 2020 (PMID:32790486)
Quintero Sierra LA et al., Tissue Eng Regen Med 2022 (PMID:35244884)
Merigo F et al., Cell Tissue Res 2021 (PMID:33159578)
人雌三醇 ( E3 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 E3 单抗包被于酶标板上，标准品和样品中的 E3与单抗结合，加入生物素化的抗人 E3 ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合，加入底物工作液显蓝色，最后加终止液硫酸，在 450nm 处测
Studies of the Ubiquitin Proteasome System
phosphatase inhibitors and 50 µM MG132 (or lactacystin) Anti‐E3 or anti‐substrate antibody Nondenaturing Triton X‐100 wash buffer (see recipe ) Tris‐buffered saline (TBS
1. Materials(1) Antibody 7E3, 2L sup grown in flasks, frozen and thawed overnight.(2) BioRad Affi-Gel Protein A MAPS II Buffers cat. #1530-6160 ($161.00)(3) 50 mM Tris pH7.8.(4) 40 g ammonium sulfate for every 100 ml Sup.(5) 4L 100 mM Tris/pH
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