- ￥1700 - 4000
- GeneTex
- 美国
- GTX112760
- 2025年07月15日
- WB, ICC/IF, IHC-P, IP
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Full length human mtTFA Recombinant protein.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX112760
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IP
- 浓度：
0.43 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
mtTFA
- 抗体英文名：
mtTFA antibody
- 抗体名：
mtTFA 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
mtTFA antibody immunoprecipitates mtTFA protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of mtTFA protein by 2 μg of mtTFA antibody (GTX112760) 12% SDS-PAGE The immunoprecipitated mtTFA protein was detected by mtTFA antibody (GTX112760) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody (GTX112760) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
TFAM antibody detects TFAM protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: TFAM stained by TFAM antibody (GTX112760) diluted at 1:1000.
mtTFA antibody detects mtTFA protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: mtTFA protein stained by mtTFA antibody (GTX112760) diluted at 1:500.
Red: Mitotracker, a mitochondria marker, diluted at 1:2000.
Blue: Hoechst 33342 staining.
Immunohistochemical analysis of paraffin-embedded human breast cancer, using mtTFA(GTX112760) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
mtTFA antibody detects mtTFA protein at mitochondria by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 2% PFA/culture medium at RT for 30 min.
Green: mtTFA protein stained by mtTFA antibody (GTX112760) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody (GTX112760) diluted at 1:1000.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody (GTX112760) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody (GTX112760) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
mtTFA antibody detects mtTFA protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
mtTFA stained by mtTFA antibody (GTX112760) diluted at 1:165.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献和实验Yan H et al., Neuropharmacology 2021 (PMID:34089729)
Yan W et al., Aging (Albany NY) 2021 (PMID:34139672)
Prieto-Carrasco R et al., Biology (Basel) 2021 (PMID:33919054)
Wang Y et al., Aging (Albany NY) 2020 (PMID:33231568)
Qiao A et al., J Cell Biol 2017 (PMID:28183717)
Miao J et al., Aging Cell 2019 (PMID:31318148)
Jinhua Miao et al., Physiol Rep 2021 (PMID:33463897)
Ren B et al., Oxid Med Cell Longev 2022 (PMID:35222795)
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