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mtTFA antibody
Full length human mtTFA Recombinant protein.
IgG
Liquid
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Polyclonal
Unconjugated
Human, Mouse, Rat
12 months from the shipping date of the product.
Human
GTX112760
Primary Antibodies
Available
GeneTex
Rabbit
WB, ICC/IF, IHC-P, IP
0.43 mg/ml (Please refer to the vial label for the specific concentration.)
mtTFA
mtTFA antibody
mtTFA 抗体
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
mtTFA antibody immunoprecipitates mtTFA protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of mtTFA protein by 2 μg of mtTFA antibody (GTX112760) 12% SDS-PAGE The immunoprecipitated mtTFA protein was detected by mtTFA antibody (GTX112760) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody (GTX112760) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
TFAM antibody detects TFAM protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: TFAM stained by TFAM antibody (GTX112760) diluted at 1:1000.
mtTFA antibody detects mtTFA protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: mtTFA protein stained by mtTFA antibody (GTX112760) diluted at 1:500.
Red: Mitotracker, a mitochondria marker, diluted at 1:2000.
Blue: Hoechst 33342 staining.
Immunohistochemical analysis of paraffin-embedded human breast cancer, using mtTFA(GTX112760) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
mtTFA antibody detects mtTFA protein at mitochondria by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 2% PFA/culture medium at RT for 30 min.
Green: mtTFA protein stained by mtTFA antibody (GTX112760) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody (GTX112760) diluted at 1:1000.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody (GTX112760) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody (GTX112760) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
mtTFA antibody detects mtTFA protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
mtTFA stained by mtTFA antibody (GTX112760) diluted at 1:165.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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