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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Goats were immunized with a highly purified mouse IgE hybridoma protein in CFA.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
- 克隆性:
Polyclonal
- 标记物:
HRP
- 适应物种:
Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Mouse
- 目录编号:
GTX77227
- 级别:
Secondary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Goat
- 应用范围:
WB, ELISA, IHC
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Goat Anti-Mouse IgE
- 抗体英文名:
Goat Anti-Mouse IgE antibody (HRP)
- 抗体名:
Goat Anti-Mouse IgE 抗体 (HRP)
- 规格:
500 μg
ELISA analysis of immunogen using GTX77227 Mouse IgE antibody (HRP).
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文献和实验Kyburz A et al., J Allergy Clin Immunol 2018 (PMID:30240703)
Kyburz A et al., Clin Exp Allergy 2017 (PMID:28802077)
Ogawa T et al., J Immunol 2020 (PMID:32690656)
Purifying and Measuring Immunoglobulin E (IgE) and Anti-IgE
to anaphylaxis. Here, we describe the history behind the IgE discovery and the protocol for purifying IgE and anti-IgE in the mouse. This is followed by our ELISA protocol for mouse IgE detection.
4-Hydroxy-2-Nonenal (4-HNE) Staining by Anti-HNE Antibody
using an anti-4-HNE monoclonal antibody (MAb) (6 –8 ) and labeled goat anti-mouse IgG antibody (9 ).
which employs goat anti-Rabbit HRP.12. Wash filter 3 times 7 minutes with 0.5% BSA in TBST.13. Perform Alkaline phosphatase reaction using NBT and BCIP substrates. I find that CREB usually comes up within several minutes of adding the AP substrates
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