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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Occular melanoma cell line V+B2.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
- 克隆性:
Monoclonal
- 标记物:
PE
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX75618
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
FACS
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
Integrin beta 1 / CD29
- 抗体英文名:
Integrin beta 1 / CD29 antibody [4B7R] (PE)
- 抗体名:
Integrin beta 1 / CD29 抗体 [4B7R] (PE)
- 规格:
100 test
FACS analysis of human peripheral blood lymphocytes using GTX75618 Integrin beta 1 / CD29 antibody [4B7R] (PE).
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文献和实验Ueda S et al., J Clin Immunol 2010 (PMID:20714794)
Itou J et al., Biochim Biophys Acta 2016 (PMID:27773610)
Su LJ et al., Sci Rep 2017 (PMID:28358111)
Western Blotting with Horseradish Pe
). 2.Aspirate excess PBS. 3.Add 1ml boiling 2X concentrated electrophoresis sample buffer (125mM Tris pH 6.8, 4% SDS, 10% glycerol, 0.006% bromophenol blue, 1.8% beta-mercaptoethanol). 4.Scrape cells from dish, transfer to a microcentrifuge tube, and boil
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Phycoerythrin conjugation protocol
Centricon 30 and Centriprep 30 centrifugal concentrators (Amicon) * Room temperature is about 22 °C Method 1. Preparation of PE 2. SPDP modification of PE 3. SMCC modification of antibody 4. DTT
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