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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
请参考说明书
- 亚型:
请参考说明书
- 形态:
液体
- 保存条件:
Aliquot and store at -20ºC
- 克隆性:
请参考说明书
- 标记物:
请参考说明书
- 适应物种:
human/mouse/rat等
- 宿主:
rabbit/mouse
- 应用范围:
ELISA,WB,IHC等
- 浓度:
请参考说明书
- 靶点:
请参考说明书
- 抗体英文名:
mAChR M2 antibody
- 抗体名:
mAChR M2 antibody
- 规格:
25μg
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文献和实验As a complement to the intracellular antibody capture method to isolate intracellular single domain antibody fragments (iDabs) from high diverse libraries, we describe here a simple mammalian two-hybrid (M2H) protocol using a “bait-prey
of TBS-mod buffer, vortexed and centrifuged for 30 seconds at 6,000 RPM(comment 4); ;Gently mix (rotating plate) the cell lysate/ChIP pulldown with the antibody-M2 beads for 2-3 hours or overnight at 4°C;Capture the Flag-antibody-M2 immunoprecipitated
Identification of a Mutant Kinase/ATP Analog Pair
Prepare the antibody for the immunoprecipitation (Harlow and Lane 1999). We use M2 agarose for FLAG-tagged ERKs. If the antibody to the epitope tag on the protein is not preconjugated, prebind the primary antibody for your epitope tag
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