- ￥5600
- GeneTex
- 美国
- GTX13593
- 2025年07月16日
- WB, ICC/IF, IHC-P, FACS, ChIP assay
- Rabbit
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human STAT5a that contains tyrosine 694. The sequence is conserved in mouse and rat.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX13593
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, FACS, ChIP assay
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
STAT5A (phospho Tyr694)
- 抗体英文名：
STAT5A (phospho Tyr694) antibody
- 抗体名：
STAT5A (phospho Tyr694) 抗体
- 规格：
50 μl
ICC/IF analysis of HeLa cells treated with 5uM of TNF-alpha for 30 minutes using GTX13593 STAT5A (phospho Tyr694) antibody. Panel e is untreated cell with no signal. Panel f is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% PFA
Permeabilization : 0.1% Trito X-100 for 10 minutesDilution: 1:250
IHC-P analysis of mouse spleen tissue using GTX13593 STAT5A (phospho Tyr694) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20
ChIP analysis of 2 million HeLa cells treated with 100ng/ml of IFN gamma for 45 minutes using GTX13593 STAT5A (phospho Tyr694) antibody. Normal Rabbit IgG was used as a negative IP control. The precipitated DNA was detected by PCR with primer set targeting to the promoter of COX1, AP1 gene as positive control target and the inactive SAT2 used as negative control target.
WB (peptide competition) analysis of 3T3-L1 cells treated with 20 ng/mL LIF for 10 minutes (Lane 2-5) using GTX13593 STAT5A (phospho Tyr694) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphotyrosine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
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文献和实验Lu JW et al., Cancer Lett 2016 (PMID:27060207)
Uchino Y et al., Cancer Cell Int 2015 (PMID:25805962)
Vazquez-Mellado MJ et al., Life Sci 2015 (PMID:26232556)
Chi M et al., Sci Rep 2016 (PMID:27605043)
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