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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Peptide mapping to the carboxy terminus of human p27.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat, Sheep
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX27961
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, IP, IHC
- 浓度:
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
p27 Kip1
- 抗体英文名:
p27 Kip1 antibody
- 抗体名:
p27 Kip1 抗体
- 规格:
1 ml
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文献和实验Bellingham M et al., Mol Cell Endocrinol 2013 (PMID:23791816)
Chen YJ et al., Cancer Lett 2012 (PMID:22266097)
Procedure Dilute p27 monoclonal antibody 1:1000 (v/v) in Carbonate Coating Buffer. Add 100 µl/well and incubate o/n @ 4°C or 1 hr @37°C. Wash 3X with TBST (pour onto plate, empty into sink, hit onto towel 3x to clear wells
-induced responses, including a down-regulation of the cyclin-dependent kinase (Cdk2) inhibitors p21CIP1 and p27KIP1 , while attachment to P-L-L did not. We conclude that engagement of the FN-receptor integrin α5β1 induces integrin signaling regulating
for determining the activity of PNAs designed to target oncomiRNAs, using as model system miR-221 and its target p27Kip1 mRNA. The effects of PNAs targeting miR-221 are here presented discussing data obtained using as model system the human breast cancer cell line
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