- ￥5600
- GeneTex
- 美国
- GTX25636
- 2025年07月11日
- WB, ICC/IF, IHC
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human EGFR that contains tyrosine 845. The sequence is conserved in mouse and rat.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX25636
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
The full-length EGFR includes the 24 aa signal peptide that is cleaved during EGFR maturation. The Tyr845 of mature EGFR is equivalent to Tyr869 of uncleaved EGFR.
- 抗体英文名：
EGFR (phospho Tyr845) antibody
- 抗体名：
EGFR (phospho Tyr845) 抗体
- 规格：
50 μl
ICC/IF analysis of HeLa cells treated with EGF (200 ng/mL for 10 min) using GTX25636 EGFR (phospho Tyr845) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% PFA
Permeabilization : 0.25% Triton X-100 for 10 minutes
WB analysis of samples using GTX25636 EGFR (phospho Tyr845) antibody. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
ICC/IF analysis of A431 cells treated with 200 ng/ml of EGF for 10 minutes using GTX25636 EGFR (phospho Tyr845) antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% PFA
Permeabilization : 0.1% Triton X-100 for 10 minutes
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文献和实验【求助】干扰一个基因表达后，EGFR的表达及tyr1173磷酸化都降低，如何分析
zxlbaby 干扰一个基因表达后，EGFR的表达及tyr1173磷酸化都降低，如何分析？能说此基因具有调节EGFR的表达和磷酸化作用吗？还是磷酸化减少是因为表达减少？郁闷中。求助。 amygdala 用WB检测以后最好用条带扫描软件做一个量化统计分析，看看总EGFR和Tyr1173降低的比例是否一致，这样就可以知道是哪种情况了。 lilili1979 同意楼上的,可以考虑用磷酸化的/总蛋白
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
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