- ￥5600
- GeneTex
- 美国
- GTX25636
- 2025年07月11日
- WB, ICC/IF, IHC
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 技术资料
- 免疫原：
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human EGFR that contains tyrosine 845. The sequence is conserved in mouse and rat.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX25636
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
The full-length EGFR includes the 24 aa signal peptide that is cleaved during EGFR maturation. The Tyr845 of mature EGFR is equivalent to Tyr869 of uncleaved EGFR.
- 抗体英文名：
EGFR (phospho Tyr845) antibody
- 抗体名：
EGFR (phospho Tyr845) 抗体
- 规格：
50 μl
ICC/IF analysis of HeLa cells treated with EGF (200 ng/mL for 10 min) using GTX25636 EGFR (phospho Tyr845) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% PFA
Permeabilization : 0.25% Triton X-100 for 10 minutes
WB analysis of samples using GTX25636 EGFR (phospho Tyr845) antibody. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
ICC/IF analysis of A431 cells treated with 200 ng/ml of EGF for 10 minutes using GTX25636 EGFR (phospho Tyr845) antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% PFA
Permeabilization : 0.1% Triton X-100 for 10 minutes
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