- ￥5600
- GeneTex
- 美国
- GTX24890
- 2025年07月12日
- WB, IHC-P
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Shc that contains tyrosines 239 and 240.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX24890
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, IHC-P
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody cross-reacts with over-expressed insulin receptor protein in CHO cells.
- 抗体英文名：
SHC1 (phospho Tyr239/Tyr240) antibody
- 抗体名：
SHC1 (phospho Tyr239/Tyr240) 抗体
- 规格：
50 μl
WB analysis of whole cell extracts (20 μg lysate) of A431 (Lane 1), treated for 10 minutes with 200 ng/ml of EGF (Lane 2), NIH/3T3 (Lane 3) and treated for 10 minutes with 200 ng/ml of EGF (Lane 4) using GTX24890 SHC1 (phospho Tyr239/Tyr240) antibody.
Dilution : 1:500
IHC-P analysis of human colon carcinoma tissue using GTX24890 SHC1 (phospho Tyr239/Tyr240) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20
WB analysis of A431 stimulated with EGF (Lane 1-4) or left unstimulated (Lane 5) using GTX24890 SHC1 (phospho Tyr239/Tyr240) antibody.
WB analysis of membrane enriched extracts (30 ug lysate) of A431 (Lane 1), A431 treated with EGF (200 ng/ml for 10 minutes) (Lane 2), A431 treated with Afatinib followed by EGF (0.5 uM for 6 hours, 200ng/ml for 10 minutes) (Lane 3), A549 (Lane 4), A549 treated with EGF (200 ng/ml for 10 minutes) (Lane 5) using GTX24890 SHC1 (phospho Tyr239/Tyr240) antibody.
Dilution : 1:500
WB analysis of 30 μg of A431 (Lane 1) treated with EGF (200 ng/ml for 10 minutes) (Lane 2), treated with Afatinib followed by EGF (0.5 uM of Afatinib for 6hrs, 200ng/ml for 10 minutes) (Lane 3), EGFR KO A431(Lane 4) treated with EGF (200 ng/ml for 10 minutes) (Lane 5), EGFR KO A431 treated with Afatinib followed by EGF (0.5 uM of Afatinib for 6hrs, 200ng/ml for 10 minutes) (Lane 6) whole cell extracts using GTX24890 SHC1 (phospho Tyr239/Tyr240) antibody.
Dilution : 1:500
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 antibody (A) and Total-p38 antibody (B). Cell Based Elisa were performed directly in the 96-well plates using Phospho-p38 and Total-p38 antibodies (C). Figure 3: JNK detection with FACECells were serum-starved for 16 hours
运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 antibody (A) and Total-p38 antibody (B). Cell Based Elisa were performed directly in the 96-well plates using Phospho-p38 and Total-p38 antibodies (C). Figure 3: JNK detection with FACECells were serum-starved for 16 hours
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