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- 规格:
100 ul
predicted_size:14.7 kDa
Uniprot ID:Q2363
description:ID2 mouse monoclonal antibody, clone OTI10C3 (formerly 10C3)
clonality:Monoclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...):Primary Antibodies
background:The protein encoded by this gene belongs to the inhibitor of DNA binding (ID) family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the ID family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene has been identified for this gene.
immunogen:Full length human recombinant protein of human ID2 (NP_002157) produced in E.coli.
recommended_dilution:WB 1:500, IHC 1:50, IF 1:50
predicted_size:14.7 kDa
buffer:PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
host_species:Mouse
applications:IF, IHC, WB
reactivities:Human, Mouse, Rat
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production
Monoclonal Antibody Production Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform
The production of monoclonal antibody by hybridoma
The production of monoclonal antibody by hybridoma fusion: Immortalization of sensitized B lymphocytes from immune mice. Overview
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