Matrices listed below were spiked with certain level of MIP-1α and the recovery rates were calculated by comparing the measured value to the expected amount of MIP-1α in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-95
92
EDTA plasma(n=5)
85-101
91
heparin plasma(n=5)
85-103
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MIP-1α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.